Journal: Cell

Article Title: ASF/SF2-regulated CaMKIIdelta alternative splicing temporally reprograms excitation-contraction coupling in cardiac muscle.

doi: 10.1016/j.cell.2004.11.036

Figure Lengend Snippet: Figure 6. Switch of CaMKII to a Neuronal-Specific Isoform in ASF/SF2-Deficient Heart

Article Snippet: NeuronCaMKII (Santa Cruz), mouse anti-phosphorylated CaMKII (Affinity 23, 33–44.Bioreagents), mouse anti-SERCA2 (Affinity BioReagents), mouse anti-RyR2 (Affinity BioReagents), and rabbit anti-phosphorylated Chen, J., Kubalak, S.W., and Chien, K.R. (1998).

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of Shenmai injection and its bioactive components following ischemia/reperfusion in cardiomyocytes

doi: 10.3892/etm.2015.2662

Figure Lengend Snippet: Effects of TSPG, Rg1 and SM on SERCA and PLB mRNA expression following I/R in cardiomyocytes. (A) SERCA and (B) PLB mRNA expression levels. Data are expressed as the mean ± standard deviation (n=9). $ P<0.01 vs. N; & P<0.01 vs. I/R + SM; *P<0.01 vs. I/R; **P<0.01 vs. I/R+TSPG. SERCA, sarco/endoplasmic reticulum Ca 2+ ATPase; PLB, phospholamban; N, normoxia; RQ, relative quantification; I/R, ischemia/reperfusion; TSPG, total saponins of Panax ginseng ; Rg1, ginsenoside Rg1; SM, Shenmai injection.

Article Snippet: Mouse anti-phosphorylated PLB (p-PLB; phosphorylated at Thr 17 and Ser 16; #8496) and rabbit anti-SERCA antibodies (#9580) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Expressing, Standard Deviation, Quantitative Proteomics, Injection

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of Shenmai injection and its bioactive components following ischemia/reperfusion in cardiomyocytes

doi: 10.3892/etm.2015.2662

Figure Lengend Snippet: Effects of TSPG, Rg1 and SM on SERCA, p-PLB, p-PLB/PLB and PLB/SERCA levels following I/R in cardiomyocytes. (A) Western blot analysis of SERCA, p-PLB, PLB and β-actin levels. (B-E) Quantification of (B) p-PLB levels, (C) SERCA levels, (D) p-PLB/PLB ratio and (E) PLB/SERCA ratio. Data are expressed as the mean ± standard deviation (n=4). $ P<0.01 vs. N; & P<0.01 and $$ P<0.05 vs. I/R + SM; *P<0.01 and # P<0.05 vs. I/R; **P<0.01 and ## P<0.05 vs. I/R + TSPG. N, normoxia; TSPG, total saponins of Panax ginseng ; Rg1, ginsenoside Rg1; SM, Shenmai injection; I/R, ischemia/reperfusion; PLB, phospholamban; p-PLB, phosphorylated-PLB; SERCA, sarco/endoplasmic reticulum Ca 2+ ATPase; RQ, relative quantification.

Article Snippet: Mouse anti-phosphorylated PLB (p-PLB; phosphorylated at Thr 17 and Ser 16; #8496) and rabbit anti-SERCA antibodies (#9580) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Western Blot, Standard Deviation, Injection, Quantitative Proteomics

Journal: Frontiers in Pharmacology

Article Title: I K1 Channel Agonist Zacopride Alleviates Cardiac Hypertrophy and Failure via Alterations in Calcium Dyshomeostasis and Electrical Remodeling in Rats

doi: 10.3389/fphar.2019.00929

Figure Lengend Snippet: Zacopride improved Iso-induced maladaptive protein alterations in rat hearts. (A) Representative western blotting images 3 days, 10 days, and 30 days after the onset of Iso infusion. (B) Dynamic changes of Kir2.1 protein expression levels. (C) Dynamic changes of p -CaMKII protein expression levels relative to total CaMKII. (D) Dynamic changes in p -RyR2 protein expression levels relative to total RyR2. (E) Dynamic changes in SERCA2 protein expression. (F) Dynamic changes of cleaved caspase 3 protein expression. All data were normalized to control. Iso, isoproterenol; Zac, zacopride; Chlo, chloroquine. Data were presented as mean ± SEM (n = 3). * P < 0.05, ** P < 0.01 compared with control. # P < 0.05, ## P < 0.01 compared with Iso+Zac.

Article Snippet: Standard western blotting were performed using respective antibodies to quantify the relative levels of Kir2.1 (mouse monoclonal anti-Kir2.1, dilution 1:1,000, Sigma; or rabbit monoclonal anti-Kir2.1, 1:1,000, Abcam), CaMKII and phosphorylated CaMKII (rabbit monoclonal anti-CaMKII, 1:1,000, Cell Signaling), SERCA2 (rabbit polyclonal anti-SERCA2, 1:1,000, Cell Signaling), RyR2-phospho S2808 or total RyR2 (rabbit polyclonal anti-( p )-RyR2, 1:1,000, Abcam), cleaved caspase 3 (rabbit monoclonal anti-c-caspase 3, 1:1,000, Cell Signaling).

Techniques: Western Blot, Expressing, Control